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REFERENCE LABORATORY Kuster SP, Coleman BL, Raboud J, McNeil S, De Serres G, Gubbay J, Hatchette T, Katz KC, Loeb M, Low D, Mazzulli T, Simor A, McGeer AJ, for the Working Adult Infuenza Cohort Study Group. Acute Care Hospital Workers and Risk of Infuenza Infection during the 2009 H1N1 Infuenza Pandemic. 2013 Emerg Infect Dis (in press) Ward BJ. Nanoparticle Vaccines for Infuenza-VLP Vaccines Made in Plants. Presented at: Seminars in Vaccine Biology, Johns Hopkins School of Medicine; April 2009; Baltimore, MD. Ward BJ. Animal and Human Studies of VLP Infuenza Vaccines Made in Plants. Presented at: Seminars in Vaccine Biology, Johns Hopkins School of Medicine; April 2009; Baltimore, MD. Brophy J, Ward BJ, Samson L, Read S, Bitnun A. Immunogenicity of AS03-adjuvanted H1N1 Pandemic Infuenza Vaccine in HIV-infected Children. In Program: 19th Canadian Association for HIV Research (CAHR); May 2010; Saskatoon, SK. Poster Abstract. Brophy J, Ward BJ, Samson L, Read S, Bitnun A. Immunogenicity of AS03-adjuvanted H1N1 Pandemic Infuenza Vaccine in HIV-infected Children. In: Abstracts of the 48th Annual Meeting of Infectious Diseases Society of America; 2010 Oct 21-24; Vancouver, BC. Poster Abstract. Ward BJ, Landry N, Tremblay S, Vezina L. VLP Infuenza Vaccines Made in Plants. Presented at: Presented at: CIHR Pandemic Preparedness Conference (PPRSI); 2010 November 12-13; Ottawa, ON. Young K, Couture M, D’Aoust MA, Lavoie PO, Vézina L, Ward BJ. Presented at: Presented at: CIHR Pandemic Preparedness Conference (PPRSI); 2010 November 12-13; Ottawa, ON. Ward BJ. Animal and Human Studies of VLP Infuenza Vaccines Made in Plants. Presented at: CAIRE Research Symposium, 2010 December 5; Quebec City, QC. Young, K. A Plant-Made Virus-Like-Particle Vaccine Expressing eGFP: A New Tool to Track Immune Responses. Presented at: CAIRE Research Symposium, 2010 December 5; Quebec City, QC. Young K, Couture M, D’Aoust MA, Lavoie PO, Vézina L, Ward BJ. A Plant-Made Virus-Like-Particle Vaccine Expressing eGFP: A New Tool to Track Immune Responses [abstract]. In: Abstracts of the 9th Canadian Immunization Conference; 2010 December 5-8; Quebec City, QC. Abstract Poster 58. Also, Can J Infect Dis Med Microbiol 2010; 21(4):173-234. Andrés Pizzorno, Xavier Bouhy, Yacine Abed, Guy Boivin. Generation and Characterization of Recombinant Pandemic Infuenza A/H1N1 Viruses Resistant to Neuraminidase Inhibitors. Poster presentation. PCIRN Annual conference Apr 19-21, 2011, Ottawa, ON. Karen K. Yam, David W. Scheifele, Brian J. Ward and the PCIRN RT-03 investigators.The Immunobiology of Oil-in-Wat er Adjuvants for Infuenza Vaccines. Oral presentation. PCIRN Annual conference Apr 19-21, 2011, Ottawa, ON. Karen Yam, Brian Ward. Immunobiology of oil-in-water adjuvants for infuenza vaccines. Presentation- 5th International Soc for Vaccines Conference, Seattle, WA, Oct 2-4, 2011. Karen K. Yam, Jyotsana Gupta, Elizabeth Allen, Brian Ward. Adjuvanted infuenza vaccines in young and aged BALB/c mice. Poster. European Congress of Immunology,Glasgow, Scotland, 5-8 September, 2012. Katie Young , Manon Couture , Marc-André D’aoust , Nathalie Landry, Louis Vézina , and Brian Ward.. Innate and adaptive immune responses to plant-based H5 VLP by murine splenocytes. Poster. European Congress of Immunology, Glasgow, Scotland, 5-8 September, 2012. Katie Young, Manon Couture , Marc-André D’aoust , Nathalie Landry Louis Vézina, and Brian Ward . Innate and adaptive immune responses to plant-based H5 VLP by murine splenocytes. Poster . Canadian Immunization Conference Dec 2-5, 2012. Wisner, A. L. S., LeBlanc, J., Hatchette, T., El-Sherif, M., and McNeil, S. Molecular Detection of Streptococcus pneumoniae in Nasopharyngeal Swabs and Deduction of Serotypes by PCR. 10th Canadian Immunization Conference, , Vancouver, BC, Canada, Dec 2-5, 2012. Wisner, A. L. S., LeBlanc, J., Hatchette, T., El-Sherif, M., and McNeil, S for the PCIRN Serious Outcomes Surveillance Network Investigators. Molecular Detection of Streptococcus pneumoniae in Nasopharyngeal Swabs and Deduction of Serotypes by Polymerase Chain Reaction (PCR). Presentation. ID Research Day, Halifax, NS, Canada, April 23-24, 2012 Yam KK, Gupta J, Brewer A, Scheifele DW, Halperin S, Ward BJ, and PCIRN (Public Health Agency of Canada-Canadian Institute of Health Research Infuenza Research Network). 2012. Unusual patterns of IgG avidity in some young children following two doses of adjuvanted pandemic H1N1(2009) infuenza vaccine. Clin Vaccine Immunol 2013 Wisner, A. L. S., LeBlanc, J., Hatchette, T., El-Sherif, M., and McNeil, S. Molecular Detection of Streptococcus pneumoniae in Nasopharyngeal Swabs and Deduction of Serotypes by PCR submitted abstract for AMMI CACMID Annual Conference, Quebec City, QC, Canada, April 4-6 2013,. MacRae C, LeBlanc JJ, McNeil SA, Hatchette TF. Determination of the viral etiology and epidemiology of infuenza-negative patients with acute respiratory disease. (Dalhousie ID research day 2014; and IWK summer studentship 2013 – winner of best research project Ward BJ, Hatchette TF and the other members of the PCIRN Reference Laboratories. Why is lab testing for infuenza so diffcult? PCIRN annual meeting, Toronto. May 27, 2013 (oral presentation) Lang A, LeBlanc J, Hatchette T, ElSherif M, McNeil S, on behalf of the PCIRN Serious Outcomes Surveillance Network investigators. Molecular detection of streptococcus pneumonia in nasopharyngeal swabs and deduction of “sero”types by polymerase chain reaction (PCR). PCRIN annual meeting, Toronto. May 27, 2013. (oral presentation) Yam K. Long-term immune responses to AS03-adjuvanted, low antigen does infuenza vaccine in BALB/c mice. PCIRN annual meeting, Toronto. May 27, 2013. (oral presentation). Yam KK, Gupta J, Brewer A, Scheifele DW, Halperin S, Ward BJ; Public Health Agency of Canada/Canadian Institute of Health Research, Infuenza Research Network (PCIRN) Rapid Trial Study 3 (RT-03) Investigators. Unusual patterns of IgG avidity in some young children following two doses of the adjuvanted pandemic H1N1 (2009) infuenza virus vaccine. Clin Vaccine Immunol. 2013 Apr;20(4):459-67 Pizzorno A, Abed Y, Bouhy X, Boivin G. Identifcation of a permissive mutation restoring the ftness of the H275Y Oseltamavir resistance mutation in 2009 pandemic infuenza A/H1N1 viruses. PCIRN annual meeting, Toronto. May 27, 2013. (poster) Retamal M., Abed Y., Corbeil J. and Boivin G. Development of monoclonal antibodies against infuenza A (H1N1)pdm09 surface proteins. PCIRN annual meeting, Toronto. May 27, 2013. (poster) Young K, Vézina L, Couture M, Arthus-Cartier G, Ward B. Plant-derived infuenza H5-based virus-like particles preferentially associate with innate phagocytic cells. PCIRN annual meeting, Toronto. May 27, 2013. (poster) Young K, Couture M, D’Aoust MA, Landry N, Ward BJ. Geneation and characterization of a plant-made infuenza H5 virus-like particle containing eGFP. (2014 submitted) Yam K, Mallet C, Burt D, Ward BJ. Immune responses to extreme low-dose infuenza antigen adjuvanted with AS03 in a BALB/c mouse model (submitted 2014) 2014 ANNUAL REPORT 17